ambion特约代理商|AM9342 Formamide (

Hybridization for Spectral Karyotyping (SKY)

Reagents dH 2O Ethanol, absolute (70%, 90%, and 100%) Formamide, deionized Ambion, Cat. No. 9342 HCL, 1N Rubber Cement SSC, 20XPreparation70% Formamide/2X SSC 20X SSC 10 ml dH 2O 20 ml deionized

Biotin labeling and hybridization of probe to chromosomes

hybridization mixture: deionized formamide  10 µl 20 X SSC  2 µg

DNase I Footprinting

0.005% (w/v) Xylene Cyanol FF 20 mM EDTA make up in deionized Formamide 0.005% (w/v) Bromophenol Blue 70% (v/v) Ethanol