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Immunofluorescence: Rabbit Anti-Murine RELMα

for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide

Purification Of Anti Peptide Antibody

the antibody can be concentrated by sweating the dialysis bags, or by spin-concentrating. Bring the azide concentration up to 0.1 and store at 4 deg C for up to three months. For longer storage freeze in aliquots and store at -80 degC or add glycerol

AbC Anti-Rat/Hamster Bead Kit

for at least 1 year.   Number of assays: Sufficient material is supplied for 100 assays based on the protocol below. Materials Required but Not Provided Rat and/or hamster antibody conjugates Phosphate buffered