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上海玉博生物科技有限公司
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文献和实验Fluorescein labeling of proteins
from free Fluorescein compounds by extensive dialysis versus PBS or by gel filtration.Concentrate with Centriprep and/or Centricon concentrators as necessary. Microfuge on high for 10 minutes and filter through 0.22 µm filter.Optionally add 0.5% azide
Fluorescein labeling of proteins
compounds by extensive dialysis versus PBS or by gel filtration. Concentrate with Centriprep and/or Centricon concentrators as necessary. Microfuge on high for 10 minutes and filter through 0.22 µm filter. Optionally add 0.5% azide as preservative
has been reported to work. -------------------------------------------------------------------------------- PROTOCOL 1. Dilute stock to 10-5 M (=10 µM ) Cardiotoxin: MW 6800 (1 mg = 1.47 x 10-7 moles) start with 1 mg of lyophilized powder
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