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文献和实验Membrane Transfer and Crosslinking for RNA
The composition of the transfer buffer is usually a 5X SSC/10 mM NaOH solution. These mildly alkaline conditions shear the RNA into smaller fragments and denature it as it is deposited onto the membrane. A brief protocol for assembly (see Figure 1
Preparation of Brain Membrane Fractions
. This consists primarily of synaptic plasma membrane.Dilute with TA to 10% sucrose (by adding 3 vol of TA), and pellet by centrifugation at 48,000 x g for 30 min.Resuspend the pellet in water or TA, estimate the protein concentration, aliquot, and store at –70℃
[分享] Strip Antibodies and Re-use Protein blots membrane
, it will be very hard to clean up). Three： Stripping of the membrane • PVDF membranes may be stripped. Nitrocellulose generally does not strip well. • Wash membrane for 30 minutes in 25 mM glycine-HCl pH 2.0 + 1% SDS with gentle shaking at room temperature
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