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文献和实验Anti-GFP/BrdU In Situ Immunostaining
Materials 24 well dishes Millicell 0.4 µm inserts (Millipore PICM01250) - coated with fibronectin Culture medium PBS Fixative: 4% paraformaldehyde in PBS (pH 7.6) Rabbit anti-GFP (Molecular Probes, A-11122) FITC-goat anti-rabbit
Stimulation of Anti-HIV-1 Cytotoxic T Lymphocytes by Dendritic Cells
molecules CD40, CD80, and CD86 (5 ,6 ), and production of cytokines such as interleukin-12 (IL-12) (7 -9 ), IL-6 (10 ), and interferon α (IFN-α) (10 ,11 ). Moreover, DC can process antigens in various forms by different pathways and induce high levels
FITC荧光二抗?――EarthOx新型DyLight 488绿色荧光
FITC荧光二抗?――EarthOx新型DyLight 488绿色荧光 FITC(Fluorescein Isothiocyanate,异硫*酸荧光素)是一种绿色荧光团,在免疫学实验里,经常被用于荧光团标记到相应的检测分子,如抗体上。FITC的纯品为黄色或橙黄色结晶粉末,易溶于水和酒精溶剂。FITC分子量为389.4,最大吸收光波长为 490~495nm,最大发射光波长为520~530nm,呈现明亮的黄绿色荧光。FITC在冷暗干燥处可保存多年,是目前应用最广泛的荧
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