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文献和实验Isolation of Microtubules (Bovine Brain)
( -aminoethyl Ether) N,N,N',N'-tetraacetic acid) 0.5 mM MgCl Adjust pH to 6.4 8 M Glycerol in MT buffer Virtis homogenizer (or equivalent) Refrigerated centrifuge GTP Bradford protein assay Procedure
DNA Delivery to Cells in Culture Using PNA Clamps
Peptide nucleic acid (PNA) is a DNA mimic in which the deoxyribose phosphate backbone has been replaced by N -(2-aminoethyl) glycine linkages. PNAs, first described by Nielsen et al. in 1991 (1 ), possess a number of useful properties
). One of the most interesting issues is the possible development of miRNA therapeutics for development of anti-cancer molecules. In this respect appealing molecules are based on peptide nucleic acids (PNAs), displaying a pseudo-peptide backbone composed of N -(2-aminoethyl
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