DAKO 代理商|R0437 Polyclonal Rabb
it Anti-Human Lambda Light Chans/RPE Rabbit F(ab)2 1ml/100T- ￥200000
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- 2025年07月16日
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上海玉博生物科技有限公司
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文献和实验IMMUNOSCREENING OF LAMBDA-GT11 BACTERIOPHAGE LIBRARIES WITH ANTIBODY PROBES
dishes of lysed E. coli to reduce anti-E. coli antibody background. These antibodies are present in all human sera, rabbit sera, and rodent sera. For each Petri dish containing solidified LB agar, mix 0.6ml of the Y1090 overnight-saturated culture with 30
Double immunohistochemistry for BCL6 and PNA for Germinal Center detection.
chamber, at RT overnight . Rabbit anti BCL-6 (Santa Cruz Biotechnology, Santa Cruz, CA: Bcl-6 (N-3): sc-858, use at 0.5 µg/ml) 5- Wash twice in TBS-T. 6- add the biotin-conjugated secondary antibody (100 to 200 µl) and incubate for 45 min
T-cell dependent antigen specific in situ staining (Pe)
HRP-conjugated secondary Ab choice: 10- add the HRP-conjugated Goat anti rabbit (Vector 50 to 100 µl, dilution 1:100) and incubate for 30’. Swine anti-rabbit (Dako) can be used at 1:300 dilution. If you use this step for tyramide
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