QIAGEN凯杰特约代理商|974912 PyroMark

Analysing DNA Methylation Using Bisulphite Pyrosequencing

tandem assays along the amplicon. The general information provided is applicable for all formats of current pyrosequencing instruments, however, a specific protocol for the PyroMark Q24 instrument is provided.

DNA甲基化检测技术全攻略

基化研究提供了新的途径。 通过准确定量单个连续的CpG 位点上的甲基化频率，焦磷酸测序本身能检测并定量甲基化水平上的细微改变。在序列延伸过程中，根据C和T的掺入量来定量确定单个位点的C-T 比例。因此，不同位点的甲基化变异就能被准确检测。由于焦磷酸测序提供了真实的序列数据，甲基化状态也就以序列形式呈现。 QIAGEN目前提供三种焦磷酸测序仪器，通量由低到高，适合不同的应用。PyroMark Q24 的强项在于可对多达24个样品进行焦磷酸测序。需要大样品量的应用更适合

2-D Polyacrylamide Gel Electrophoresis

(Red/+)end and also that the anode and cathode electrode ridges are in the correct orientation. 12.Overlay liberally with DryStrip Cover fluid between the immobilon strips and outside the electrodes . 13.Electrophorese for 36-48 hrs,using