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上海玉博生物科技有限公司
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文献和实验Radioactive DNA Fragmentation Assay
for a certain time (e.g. 48h) at 37�; Harvest cells: pipet supernatant into another plate, pipet 50 � of Trypsine/EDTA to the cells, incubate 37� until all cells are detached, pipet the supernatants back to the corresponding wells containing
transfers. For gels to be stained, stain for 20 min with Coomassie blue solution (see below), then destain in strong destain (50% methanol, 10% acetic acid) 20 min and leave in 10% acetic acid.Reagents for SDS PAGEStock solutions:A. Acrylamide=30 g
Protocol for vacuum-infiltration transformation of Arabidopsis thaliana
the herbicide solution on the plantlets. Efficient recovery was achieved using the following protocol : seeds (up to 1 g : approximately 50,000 seeds) are mixed with 4-5 volumes of fine sand and sown on 30 cm X 30 cm trays containing medium sand. The trays
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