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文献和实验Fastfilter Plasmid Maxi Kit Spin Protocol
as above and discard flow-through. 14. Centrifuge the empty capped column for 10 min at maxi speed (no more than 6,000 x g) to dry the column matrix. DO NOT skip this step - it is critical for removing traces of ethanol that may otherwise interfere
Plant DNA methylation analysis by bisulfite genomic sequencing
): T2f 5' AYG YGA TAG AAA AYA AAA TAT AG 3' T1r 5' CCT TTA CTC ATT RTT ATA TCT CC 3' Primers were ordered from Metabion "OPC-purified" as stock solutions of 100pmol/µl.
Stool DNA Protocol (for pathogen detection)
capable of at least 13,000 x g 2. Nuclease-free 1.5 mL or 2 mL microfuge tubes 3. Water bath or heating block preset to 65°C and 70°C 实验步骤 1. Weigh up to 50-100 mg of stool sample in a 2 mL centrifuge tube containing 200
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