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上海玉博生物科技有限公司
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文献和实验Analysis of ES Cell Clones by Mini-Southern
Analysis of ES Cell Clones by Mini-Southern Initial Cloning 1. Aspirate selective media off the plates containing colonies of interest. Wash the plates twice with PBS. After aspirating the second wash, add enough PBS to cover
【资源】western blot 磷酸化蛋白检测裂解液完整配方
50ul(2.5M) 100ul 10uM ALLN 5ul(10mM) 10ul 10uM ALLM 5ul(10mM) 10ul 0.1mM Sodium Orthovanadate 5ul(100mM) 10ul Protease Inhibitor Cocktail 5ul 10ul Phosphatase Inhibiror Cocktail 5ul 10ul Complete Tablet 1/2 1(mini
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
:chloroform:isoamyl alcohol, UltraPure (25:24:1 v/v/v; Invitrogen) Phosphate-buffered saline (PBS; GIBCO 20012) Protease inhibitor cocktail (Sigma P8340) Proteinase K (10 mg/mL in TE buffer, pH 8.0; Invitrogen) PureLink HQ 96 Mini
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