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文献和实验BAC Modification Using a RecA Expressing Shuttle Vector System
transgenic ES cell lines by liposome-mediated transfection (6 ). The most widely used BAC vectors such as pBeloBAC11 or pBACe3.6 (7 ,8 ) lack the selection markers necessary for easeful transfection study in mammalian cells. To use such BAC clones
containing the human Lhcgr gene into a 170 kb BAC clone comprising the entire mouse orthologue. The methodology is based on the rationale to introduce a counter-selection cassette flanked by unique restriction sites and HAs for the insert, into the vector
Construction of Gene-Targeting Vectors by Recombineering
region is then retrieved from the BAC into PL611, which contains the Amp R gene. (D ) The Bsd cassette is then replaced by a reporter (i.e., the lacZ reporter cassette) by a simple restriction digestion and ligation process. The final targeting vector
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