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上海玉博生物科技有限公司
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文献和实验Assembly of Nucleosomal Templates by Salt Dialysis
Because it is convenient to assemble nucleosomal templates through salt dialysis, large amounts of chromatin complexes can be made easily and in a short amount of time. This unit includes instructions for the various salt dialysis schemes (step versus gradient
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
centrifugation (3000-5000 x g (6600 rpm) for 1 min)), and remove supernatant (use gel loading tips to completely remove supernatant). (23) Add 1 ml of ice-cold Low Salt Immune Complex Wash Buffer (EZ ChIP kit). Resuspend the packed agarose beads by gentle
DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
temperature for 5 minutes. 9. Centrifuge at maximum speed ($13,000 x g) at 4°C for 10 minutes to precipitate Nucleic acid. Discord the supernatant. 10. Wash the nucleic acid pellet by adding 400 μL of 70% ethanol into the tube. Vortex the tube
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