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文献和实验8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
secondary antibody review -- data from 99 publications
IgG Alexa Fluor 488 1:250 Molecular Probes 10 goat Cy3 immunocytochemistry detect NMDA receptors in HEK293 cells Jackson ImmunoResearch Laboratories 10 FITC immunocytochemistry 1:100 detect phospho-CREB in human colonic
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