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Specificity Profiling of Protein‐Binding Domains Using One‐Bead‐One‐Compound Peptide Libraries

, L., Kim, S., Carpino, N., Daniel, J.L., Kunapuli, S.P., Tsygankov, A.Y., and Pei, D.H. 2010. Determination of the substrate specificity of protein‐tyrosine phosphatase TULA‐2 and identification of Syk as a TULA‐2 substrate. J. Biol. Chem. 285:31268

Appendix H: Radioactive tracers

     The use of radioactive tracers in cell research is an effective and safe means of monitoring molecular interactions. There is simply no other technique which allows the precision and specificity of radioactive tracers

Mapping Protein Distributions on Polytene Chromosomes by Immunostaining

fluorescence-conjugated secondary antibody solution to the slide, and cover with a coverslip. Incubate for 1 h at room temperature in a humid chamber. For double-labeling experiments, both secondary antibodies of the appropriate type can be applied