clontech特约代理商|631232 Lenti-X C

Lenti-Virus Protocol

Lenti-Virus Protocol 张端午 A. For packing virus in 6-well plate: (if in 12-well plate, reduce all to 1/2) 1. Mix the following plasmids in a 1.5 ml eppendorf tube, 1.5 μg Lenti-vector + 1.5 μg packing plasmids = 3 μg total 0.5:0.3:0.2

Clonality - X Chromosome Inactivation Assay

can utilize X chromosome inactivation (methylation) to determine the clonality status of a tumor or premalignant lesion in females. The technique is based on a methylation-sensitive restriction enzyme and analysis of a polymorphic locus on the X chromosome

交联染色质免疫共沉淀（X-ChIP）实验设计

。加入甘氨酸可以消除甲醛使交联反应终止。1.1.准备两个长满细胞的150cm2的细胞培养皿（1*107-5*107 个细胞/皿）。将甲醛直接滴入PBS洗过的细胞培养皿中，使其终浓度为0.75%，然后在室温缓慢旋转10分钟，使蛋白和DNA发生交联。1.2 加入甘氨酸使其终浓度为125mM,在室温晃动孵育5分钟。1.3 使用10ml预冷PBS清洗细胞2次1.4 使用细胞刮将细胞收获放入 5ml 预冷PBS中，并转入50ml的管子。1.5.在皿里加入3mlPBS，将剩余的细胞转移到50ml管子里1000g