orgentec特约代理商|OGR618 Anti-PR3

Heparan Sulfate Proteoglycan-Mediated Polyamine Uptake

variable fragment (scFv) anti-HS antibodies to block HSPGs at the cell surface. Finally, we provide a protocol for the quantitative verification of loss or reduction of cell surface HSPGs and a detailed description of polyamine uptake measurement.

酵母免疫荧光染色--Immunofluorescent Staining of Yeast:SDS Permeabilization Metho

KH2PO4  with  450  mls  of  ddH2O   4)  37%  Formaldehyde   5)  KS  Buffer:  0.1  M  KPO4  pH  7.5/1.2  M  Sorbitol   6)  Zymolyase  100T:    5  mg/ml  solution  in  0.1M  KPO4/0.5%  β- mercaptoethanol   7)  β-mercaptoethanol   8)  HS  Buffer: 

Intracellular Immunofluorescent Staining for Flow Cytometry

. In this protocol, cells are first activated in vitro , stained for surface antigens as in the surface antigen protocol, then fixed with paraformaldehyde to stabilize the cell membrane and permeabilized with the detergent saponin to allow anti-cytokine antibodies