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文献和实验Vybrant® DyeCycle Green and Orange Stains
DyeCycle™ Staining Protocol This basic protocol is optimized using Jurkat cells suspended in complete medium (RPMI/10% fetal bovine serum) and stained with Vybrant® DyeCycle™ stain at 37?C. Remove the Vybrant® DyeCycle™ Green stain
海洋微生物的培养基：Marine Agar with Sulfur(ATCC Medium 1922)
1.6mgpH 7.6 ± 0.2 at 25°CPreparation of Sulfur: Autoclave sulfur for 15 min at 0 psi pressure–100°C on three successive days.Preparation of Medium: Prepare anaerobically under a gas phase of 80% N2 + 10% CO2 + 10% H2.Add components, except sulfur
Vybrant® DyeCycle Violet Stain
This basic protocol is optimized using Jurkat cells suspended in complete medium (RPMI/10% fetal bovine serum) and stained with Vybrant® DyeCycle™ Violet stain at 37?C. Remove the Vybrant® DyeCycle™ Violet stain from the refrigerator and allow
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