NEB特约代理商|M0293S Exonuclease I（

E. coli K-12限制外源DNA

或EcoRI位点修饰的DNA。除了限制M.SssI修饰的DNA，McrA还会限制HpaII甲基化酶(5′Cme CGG)修饰的DNA(2)，而McrBC会限制含5′Rme C序列的DNA(2,3,13)。McrA和McrBC不区分5-甲基胞嘧啶和5-羟甲基胞嘧啶；McrBC还会限制在适当序列中包含N4-甲基胞嘧啶的DNA(3)。Mrr限制多种腺嘌呤甲基转移酶以及几种5-甲基胞嘧啶甲基转移酶修饰的DNA，但还没有推导出共有顺序(4，6)。 几乎所有实验室的E．coli 菌株都是野生分离株K-12或B

Detection and Quantitation of RecBCD Enzyme (Exonuclease V) Activity

the recombination-initiating function of RecBCD (1 ,3 ). The enzyme in Escherichia coli and other bacteria consists of three protein subunits encoded by the recB, recC, and recD genes, whereas some bacteria (e.g., Bacillus subtilis, Lactococcus lactis) produce

Simple Cloning and DNA Assembly in Escherichia coli by Prolonged Overlap Extension PCR

can be applied to a few common Escherichia coli hosts (e.g., BL21(DE3), DH5α, JM109, TOP10). The protocol includes three steps: (a) linear DNA fragments (i.e., the insert DNA and the vector backbone) with two overlap ends were generated by regular high-fidelity