Serva特约代理商|1745401 胶原酶NB4 I/II

Overview of Formation of G‐Quadruplex Structures

Figure 17.2.1 (A ) Formation of a G‐tetrad. (B ) Anti (left) and syn (right) guanine conformations. (C ) Orientations of G‐strands. (i) All strands in the same direction. (ii) Three strands in one direction, and the fourth in the opposite direction

小鼠脾脏树突细胞的分离－胶原酶消化制备脾脏细胞悬液

辅助方案： 胶原酶消化制备脾脏细胞悬液 实验材料： 1. 4000U/ml胶原酶D，融化后置于冰上 2. HBSS，已灭菌，含Ca+ 、Mg2+ （购置于Life Technologies） 3. 小鼠脾脏 4. 皮下注射针，22G×11/2 in内径（Becton Dickinson） 5. 10ml、5ml一次性注射器（如Becton

Differential Display of RNAs

, T12 MG, T12 MT ; where M is A,C or G) dilute DNA-free RNA to 0.1 µg/µl with H2 ODEPC , keep on ice set up cDNA synthesis reaction for each degenerate anchored oligo(dT) primer set: 4 µl 5 x reverse transcriptase buffer, 2 µl DTT