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文献和实验one would consider, is that the fetal bovine serum content is too low, but our media supports the growth of Sp2/0 and established hybridoma cells very well. The problem here appears to be the CO2 content. Therefore, high cell density maintains the CO2 at a higher
Procedures for In Vitro Production of Bovine Embryos
Procedures for In Vitro Production of Bovine EmbryosThe procedures for in vitro production [IVP; i.e. in vitro maturation (IVM), in vitro fertilization (IVF), and in vitro culture (IVC)] of embryos described here are based on procedures developed
Healthy Sp2/0 cells should be rapidly growing by this time. Sp 2/0 cells should be started about two weeks before the cell fusion. Every two days, they should be centrifuged at a 64.4 xg on the IEC clinical centrifuge (in 50 ml tubes
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