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文献和实验therapeutics aimed at treating CNS disorders. Curr. Protoc. Pharmacol. 58:5.62.1?5.62.22. © 2012 by John Wiley & Sons, Inc. Keywords: abuse liability; self?administration; drug discrimination; locomotor sensitization; rat; mouse
Procedures for In Vitro Production of Bovine Embryos
with OMM + supplement microdrops (pre-equilibrated) Bench paper to cover surface 25 ml pipet automatic pipettor Mesh or styrofoam on counter X-plate Integrid petri-dish100 μm Falcon Cell Strainer50-ml Centrifuge Tubes, self standing with plug seal cap
Adult cattle serum as supplemental nutrient in lymphocyte cultures for chromosomes studies
) is separated and transferred in independent sterile tubes with all careful precautions. These tubes are centrifuged at 3000 rpm for 20 minutes to get rid of cells if any. The tubes containing sera are kept in a water bath already attained temperature at 56° C
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