Cytoskeleton特约代理商|BK011P Tubul
in polymerization assay using >99% pure tubulin, fluorescence based (BK011P)- ￥3000
- Merck Millipore
- LSKMAGG10
- 2025年07月13日
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文献和实验the resulting pellets in 0.25 x V2 of cold PM buffer, incubate at on ice for 30 min and centrifuge again (steps 11-15). 18. The resulting cold supernatant is ~90% pure tubulin and is called twice-cycled tubulin
Labeling Tubulin and Quantifying Labeling Stoichiometry
after the labeling reaction by one or more cycles of polymerization and depolymerization. I. Solutions & Supplies II. Labeling Protocol III. Quantifying Tubulin Concentration and Labeling Stoichiometry IV. Using Labeled Tubulins V
Isolation of Microtubules (Bovine Brain)
minutes at 4° C for further clarification. Collect 10 ml of the supernatant and determine the protein content of your sample using the Bradford protein assay (Appendix G ). Use bovine serum albumin or lysozyme for establishing a standard
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