signagen特约代理商|SL100489 GenJet™

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™

Construction and Characterization of Adenovirus Vectors

from Step 13) with 2 µL of PacI in a total volume of 50 µL overnight at 37°C. 16. Mix 40 µL of PacI-digested DNA with 360 µL of MEM and 16 µL of transfection reagent in a 5-mL tube. Vortex vigorously for 10 sec. 17. Allow

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications

(Figure 1a). Part II Triple immunoprecipitation: H3K27 ChIP/-flag-DNMT3A/biotin-RNA A total of 4.0×106 293T cells are plated out and 24 hours later transfected plated (~70% confluent at the time of transfection) with the flag-tagged protein