signagen特约代理商|SL100489 GenJet™
In Vitro DNA Transfection Reagent (Ver. II)|GenJet(II)体外DNA转染试剂- ￥3000
- NU-CHEK
- T-135-100mg
- 2025年07月16日
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文献和实验with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™
Construction and Characterization of Adenovirus Vectors
from Step 13) with 2 µL of PacI in a total volume of 50 µL overnight at 37°C. 16. Mix 40 µL of PacI-digested DNA with 360 µL of MEM and 16 µL of transfection reagent in a 5-mL tube. Vortex vigorously for 10 sec. 17. Allow
(Figure 1a). Part II Triple immunoprecipitation: H3K27 ChIP/-flag-DNMT3A/biotin-RNA A total of 4.0×106 293T cells are plated out and 24 hours later transfected plated (~70% confluent at the time of transfection) with the flag-tagged protein
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