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文献和实验by the addition of polyethylene glycol and collected by centrifugation. The DNA pellet is resuspended in a buffer containing cesium chloride and ethidium bromide, which is loaded into polyallomer tubes and subjected to ultracentrifugation overnight. The ethidium
(all till the last drop!!!)7、gently break the pellet by tapping of the bottom of the tube.8、place the tube on 37℃ water bath; add slowly, dop by drop 1 ml of pre-warmed (37℃) 50% PEG 1500 (polyethylene glycol, Roche, Germany) continously stirring the cells
Fusion is an important procedure to produce monoclonal antibodies (MoAb).
the supernatant (SN) with a Pasteur pipette (all till the last drop!!!) gently break the pellet by tapping of the bottom of the tube. place the tube on 37ѓC water bath; add slowly, dop by drop 1 ml of pre-warmed (37ѓC) 50% PEG 1500 (polyethylene
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