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上海玉博生物科技有限公司
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文献和实验, the more concentrated the peak of elution of tubulin protein. DAY 2: Cycling preparation of MT protein. Keep the brains in an evacuated plastic ziplock bag buried in ice from the time of slaughter during transport back to the laboratory.
the resulting pellets in 0.25 x V2 of cold PM buffer, incubate at on ice for 30 min and centrifuge again (steps 11-15). 18. The resulting cold supernatant is ~90% pure tubulin and is called twice-cycled tubulin
of tubulin protein. DAY 2: Cycling preparation of MT protein. Keep the brains in an evacuated plastic ziplock bag buried in ice from the time of slaughter during transport back to the laboratory. In a 4oC cold room, carefully and thoroughly
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