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文献和实验Plant Plasma Membrane Protein Extraction and Solubilization for Proteomic Analysis
The plasma membrane (PM) exists as the interface between the cytosol and the environment in all living cells and is one of the most complex and differentiated membrane. The identification and characterization of membrane proteins
Imaging Plasma Membrane Proteins by Confocal Microscopy
Confocal microscopy has become a widely used method in the study of plasma membrane proteins. A Medline search for the terms “plasma membrane” and “confocal” returns over 1300 references since 1966. Of these, over 1000 references appeared
Protocol to Enrich and Analyze Plasma Membrane Proteins
of the membranes with sublytic concentrations of a detergent and enrichment of the plasma membranes by a density gradient fractionation. Fluorometric assays for protein content and plasma membrane marker activity allow calculation of the yield and extent of plasma
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