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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
INTRODUCTION The formidable size and structure of polytene chromosomes allow mapping of chromosomal protein distributions at very high resolution. This protocol describes the preparation of polytene chromosomes from Drosophila larvae
Solid Phase Assays for Studying ECM Protein-Protein Interactions
of an enzyme-linked immunosorbent assay (ELISA) plate (solid phase). The plate is then blocked using bovine serum albumin (BSA), and biotin-labeled protein B (here termed ’ligand’) is added. After washing the wells to remove unbound ligand, bound ligand
antigen. This can particularly occur if the blocking reagent mainly consists of molecules that are considerably larger than the coated antigen, for example the commonly used protein BSA. This would lead to false negative results, the classification
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