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文献和实验Fast and reliable mini-prep RNA extraction from Neurospora crassa
, mycelia from plates (4 days old) and single colonies of N. crassa grown on a sorbose plate. With this protocol, from 20 to 300 mg (dry weight) of mycelium, grown in liquid media or on plates, yielded between 200 µg and 3 mg of RNA depending on the growth
Evaluating Modulators of “Regulator of G‐protein Signaling” (RGS) Proteins
?protein Signaling? (RGS) proteins constitute a class of intracellular signaling regulators that accelerate GTP hydrolysis by heterotrimeric G? subunits. In recent years, RGS proteins have emerged as potential drug targets for modulation by small molecules
lution of GST-Fusion Protein from Glutathione Agarose
lution of GST-Fusion Protein from Glutathione Agarose 1. Wash the GST-fusion protein glutathione-agarose complex once with 1% Triton X-100 in PBS. 2. Resuspend the complex in 1% Triton X-100 in PBS. 3. Prepare a small column
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