SERVA特约代理商|4286602 SERVALYT™ P

蛋白偶联到磁性MagPlex™微球的方法

Monobasic Sodium Phosphate, pH 6.2 by vortex and sonication for approximately 20 seconds. 9.Add 10 μL of 50 mg/mL Sulfo-NHS (diluted in dH2 0) to the microspheres and mix gently by vortex. 10.Add 10 μL of 50 mg/mL EDC (diluted in dH

ZOOM® IPGRunner™系统：简化的双向电泳（2D gel eletrophoresis）

时间和技术专家的建议。 ZOOM® IPGRunner™系统提供了一种进行等电聚焦实验的新方法，大大地简化和加速了进行2D分离的进程。在一个一次性实验的夹盒（cassette）中的7cm固定pH梯度（IPG）胶条上进行等电聚焦，ZOOM® IPGRunnerTM夹盒(ZOOM® IPGRunner™ cassette)可以容纳多达6个胶条，并且不需要矿物油覆盖。胶条直接在夹盒中水化，使用一个特殊设计电泳核心的垂直电泳微槽（mini-cell）进行聚焦。仪器

Immobilized pH gradient (IPG) as first dimension

, 0.8% resolytes 4-8 and a trace of bromophenol blue. To achieve reswelling and simultaneous loading of the sample, the entire samples were pipetted into the grooves, narrow (1 pH unit) or wide range IPG strips (3.5-10 NL, 18 cm from Pharmacia Biotech. or home