goldbio特约代理商|MUG1 MUG

GUS报告基因的定性和定量检测

2. 染色步骤 （1）染色：加入适量配制好的GUS 染液于24孔板的孔中，将待测样品浸到GUS染液中，将24孔板置于37℃保温箱中放置6 h。 （2）漂洗：先后用50%，70%，100%的乙醇漂洗样品，每次浸泡5分钟。 （3）脱色：加入100%乙醇浸泡直至完全脱色。 （4）记录：在体视显微镜下拍照记录。 二、GUS报导基因的定量检测 GUS 能与底物MUG（分子量352.3，4-甲基伞型酮-β-葡萄糖醛酸苷，4-methylumbelliferylβ-D-glucuronide

Rapid Detection of Fetal Mendalian Disorders: Tay-Sachs Disease

-glucosamine (4-MUG), and the DNA-based analysis involves testing for the presence of specific known mutations in the α-subunit gene of Hex A. Prenatal diagnosis of Tay-Sachs disease is accomplished within 24–48 h from sampling. The preferred strategy

Integrative Food Grade Expression System for Lactic Acid Bacteria

into an intergenic chromosomal location downstream of lacZ (LBA1462), encoding a β-galactosidase. The transcriptional activity of integrated gusA3 was evaluated by GUS activity assays using 4-methyl-umbelliferyl-β-d -glucuronide (MUG) and was determined