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文献和实验Triglyceride Lipase Assays Based on a Novel Fluorogenic Alkyldiacyl Glycerol Substrate
Lipases are responsible for extracellular degradation and intracellular metabolism of lipids in animals, plants, and microorganisms. The conformational motif of all lipases whose three-dimensional structure has been determined
Developing Fluorogenic RNA-Cleaving DNAzymes for Biosensing Applications
) because no naturally occurring DNAzyme has been discovered. Several IVS studies have led to the isolation of many RNA-cleaving DNAzymes (RNase DNAzymes), which catalyze the transesterification of a phosphodiester linkage in an RNA substrate, resulting in its cleavage
, and bronchoalveolar lavage, in which a multitude of hydrolases act in concert. This unit describes a protocol to map the global proteolytic substrate specificities of complex biological samples using a concise combinatorial library of internally quenched fluorogenic
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