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PriCells: Methods of Isolation and Culture of Human Hepatocytes

. An aliquot of trypan blue solution (50 μL) should be mixed with the human hepatocyte suspension (250 μL) and allowed to stand for 2 min at room temperature. The cell suspension should then be analyzed using an improved Neubauer counting chamber

Metallomics Using Inductively Coupled Plasma Mass Spectrometry

Plasma Mass Spectrometry (ICP?MS) is a highly sensitive elemental analysis technique that has been widely applied in many fields. Here we describe applications using a broad?scale approach to examine metal usage in biology. These protocols address

Isolate or Deplete Human CD4+ T Cells - Whole Blood, Buffy Coat, MNC

一、实验原理 Dynabeads are mixed with the cell sample in a tube. The Dynabeads will bind to the target cells during a short incubation, and then the bead-bound cells are separated by a magnet. Positive isolation – discard the supernatant and use