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上海玉博生物科技有限公司
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文献和实验Chromatin Immunoprecipitation (ChIPs) Protocol (Farnham Lab)
at 4 0 C for at least 3 hours. Overnight is fine. If you are using monoclonal antibodies, add 1 ug of an appropriate secondary antibody and incubate for an additional 1 hour. Day 2 14. Add 10 uls of blocked Staph A cells
Chromatin Immunoprecipitation (ChIPs) Cloning Protocol (Farnham Lab)
inhibitors IP Dilution buffer 0.01% SDS 1.1% Triton X 100 1.2 mM EDTA 16.7 mM Tris-Cl pH 8.1 167 mM NaCl 1X Dialysis buffer 2 mM EDTA 50 mM Tris-Cl pH 8.0 0.2
Chromatin Immunoprecipitation (ChIPs) Protocol for Tissues(Farnham Lab)
buffer 5 mM PIPES pH 8.0 85 mM KCL 0.5% NP40 protease inhibitors Nuclei Lysis buffer 50 mM Tris-Cl pH 8.1 10 mM EDTA 1% SDS protease inhibitors IP Dilution buffer 0.01% SDS 1.1% Trition X 100
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