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文献和实验secondary antibody review -- data from 99 publications
whether any cell surface DFz2 had been internalized 4 flow cytometry localize of GPR30 in the endoplasmic reticulum 5 rhodamine immunocytochemistry detect kaposin B in SLK endothelial cells 6 IgG IgG2b Alexa Fluor 647 flow
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
serum, obtained from the same species as the secondary antibody. Depending on the method employed, use anti-rabbit IgG (Fc) horseradish peroxidase (HRP) conjugate (1:100 dilution), a biotin-conjugated secondary antibody, or a fluorescence-conjugated
Protocol for intracytoplasmic staining of cytokines for FACS analysis
. 2) Stimulate cells for 2 hours in vitro with 50 ng/ml PMA (Sigma, P8139) + 500 ng/ml ionomicin (Sigma I0634) in phenol red free RPMI 1640 medium + 10% fetal calf serum at 37 deg C. 3) Add Brefeldin A (Sigma, B7651) to 10mg/ml for a further 2 hr
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