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文献和实验Western Blotting with Horseradish Peroxidase Conjugates
several times through a 26-gauge needle. Centrifuge the sample for 5 minutes to pellet insoluble material, then discard pellet. Dilute an aliquot of the cell lysate sample at least 10-fold for the BCA (Pierce) protein concentration assay (SDS
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
serum, obtained from the same species as the secondary antibody. Depending on the method employed, use anti-rabbit IgG (Fc) horseradish peroxidase (HRP) conjugate (1:100 dilution), a biotin-conjugated secondary antibody, or a fluorescence-conjugated
• stabilizer soln (10% sodium thiosulfate+0.1N NaOH) • IODO-BEAD™(PIERCE) • Hamilton microsyringe (model 702, 25µl, needle gauge 22S, point style #2) • desalting column (Bio-rad DG-10 etc.) • column stand For procedure 2 • PBS
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