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文献和实验Protocols for ET recombination
. We routinely put loxP or FRTs (each 34 bps) here, for subsequent recombination by Cre or FLP recombinases respectively. PCR reaction and recipient plasmid DNA preparation The oligo powder (40 nmol without FPLC purification) was dissolved in 500 μl of dH
Protocols for ET recombination
can help, especially for shorter primers. （3）Between these two regions, you can stick whatever you like - within the limits of oligo synthesis. We routinely put loxP or FRTs (each 34 bps) here, for subsequent recombination by Cre or FLP recombinases
Protocols for ET recombination.
synthesis. We routinely put loxP or FRTs (each 34 bps) here, for subsequent recombination by Cre or FLP recombinases respectively. The oligo powder (40 nmol without FPLC purification) was dissolved in 500 ul of dH2O. Extract oligo solution
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