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- BPS Bioscience
- 79797
- 2025年07月16日
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FLT3 Kinase Assay Kit
Catalog # 79797
Size: 96 reactions
Description
The FLT3 Kinase Assay Kit is designed to measure FLT3 kinase activity for screening and profiling applications using ADP-Glo®
Kinase Assay as a detection reagent.
MBP (Myelin Basic Protein) is a non-specific protein substrate that is used as a "universal substrate" for many in-vitro kinase
activity assays. This protein is targeted by many serine/threonine kinases at conserved amino acids. We use the dephosphorylated
version of the MBP substrate in our assays to determine the kinase-mediated phosphorylation of MBP. Our assays are not
suitable for studying autophosphorylation of the kinase due to the presence of the MBP substrate.
Need us to run inhibitor screens or profile your compounds against FLT3? Check out our Kinase Screening Services.
SynonymsFms-Related Tyrosine Kinase 3 Ligand, FLT3L, CD135L
ACHE Colorimetric Assay Kit
Catalog # 82281
Size: 96 reactions
Description
The ACHE Colorimetric Assay Kit is designed to measure the activity of ACHE (acetylcholinesterase) for screening and profiling
applications. The ACHE colorimetric reaction is based on a modified Ellman’s method, using the alternative substrate acetylthio
choline iodide (ATCI) and 5,5’-dithio-bis-2-nitrobenzoic acid (DTNB) to quantify amount of thiocholine produced from the
hydrolysis of ATCI by ACHE. This assay kit comes in a convenient 96-well format, with purified ACHE enzyme (amino acids
32-614), ATCI, DTNB and assay buffer for 100 enzyme reactions. The absorption intensity of DTNB adduct (λ=410 nm) is used
to measure the amount of thiocholine product generated, which is proportional to ACHE activity.
Need us to run inhibitor screens or profile your compounds against ACHE? Check out our Metabolic Enzyme Screening Services.
SynonymsAcetylcholine receptor subunit epsilon, CHRNE, ACHRE
Cathepsin D Inhibitor Screening Assay Kit
Catalog #82141
Description
The Cathepsin D Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin D for screening and
profiling applications. The Cathepsin D assay kit comes in a convenient 96-well or 384-well format, with enough recombinant
human Cathepsin D (amino acids 21-412), its substrate, and Cathepsin buffer for 96 or 384 reactions. This kit contains Pepstatin
A as internal control.
Figure 1: Illustration of the assay principle.
The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent substrate from the quen
cher. Fluorescence intensity increases proportionally to the activity of the protease.
Need us to run inhibitor screens or profile your compounds against Cathepsin D? Check out our Protease Screening Services.
Chemi-Verse™ YES1 Kinase Assay Kit
Catalog #82556
Size: 96 reactions
Description
The Chemi-Verse™ YES1 Kinase Assay Kit is designed to measure YES1 (Yamaguchi sarcoma viral oncogene homolog 1) tyrosine
kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient
96-well format, with enough purified YES1, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
Need us to run inhibitor screens or profile your compounds against YES1? Check out our Kinase Screening Services.
SynonymsProto-oncogene c-Yes, p61-Yes, Tyrosine-protein kinase Yes, YES1
COX1 Inhibitor Screening Assay Kit
Catalog #82203
Size: 96 reactions
Description
The COX1 Inhibitor Screening Assay Kit is designed to measure COX1 (cyclooxygenase 1) activity for screening and profiling
applications. The assay kit comes in a convenient 96-well format, with enough purified recombinant COX1, Amplex™ Red,
Arachidonic Diluent, COX Assay Buffer, and 100% ethanol for 100 enzyme reactions.
STK3 (MST2) Kinase Assay Kit
Catalog #82140
Size: 96 reactions
Description
The STK3 (MST2) Kinase Assay Kit is designed to measure STK3 (serine/threonine-protein kinase 3) kinase activity for screening
and profiling applications using Kinase-Glo® MAX as a detection reagent. The assay kit comes in a convenient 96-well format,
with enough purified recombinant STK3 (MST2) kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
SynonymsKRS1; MST2; FLJ90748
TWO-Step Luciferase (Firefly & Renilla) Assay System
Catalog #60683
Description
The TWO-Step Luciferase (Firefly & Renilla) Assay System is designed for high throughput, rapid quantitation of both Firefly and
Renilla luciferases in a single mammalian cell culture sample. It comes with enough reagents for 100 assays of 100 μl each in a
96-well plate.
This assay system has several relevant features:
Sensitive – highly sensitive detection of Firefly and Renilla luciferase activity.
Stable – the luciferase signal output is stable for more than one hour, providing incubation time flexibility.
High-throughput – its simple homogeneous protocol minimizes handling steps and supports high-throughput screening
applications.
Compatibility – works with a variety of common media containing up to 10% serum and phenol red.
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文献和实验breast epithelial MCF-10A cells. 科学问题：双酚 S (BPS) 与双酚 A (BPA) 具有相似的雌激素效应。考虑到 BPS 的内分泌干扰效应，本研究采用基于质谱的代谢组学和定量蛋白质组学方法，研究了 BPS 对正常人乳腺上皮细胞株 MCF-10A 的影响。 研究内容 表型实验：暴露于 BPS 达 24 小时后，MCF-10A 细胞的增殖以一种刺激的方式发生改变，在剂量为 1μM 时增殖率最高。 蛋白质组分析：总共有 200 种蛋白质被鉴定为在暴露于 BPS
师从施一公，发表 6 篇Science/3篇 Cell，她被誉为「世界最具潜力女科学家」| 论文盘点
snRNA 的环 I 锚定，而内含子的 5ʹ剪接位点（5ʹSS）和分支点序列分别被 U6 和 U2snRNA 特异性识别。除了催化金属离子的配位作用外，Prp8 催化空腔中的 RNA 元件大多为催化作用做好了准备。催化潜伏阶段由包围 BPS 的 SF3B 复合物以及掩盖 5ʹ外显子- 5ʹSS 连接的剪接因子 Cwc24 和 Prp11 维持。这个结构与前面确定的结构一起勾勒出了前 mRNA 剪接反应的分子框架。 Bact 复合物的催化潜伏期（图片来源：Science） 3. Structure
相关专题 验证引物我们做实验，有时会用到别人已经用过的引物，但如何才能知道这个引物是否正确呢？我通常利用prime 5.0来验证引物。下面我用常用的内参GAPDH将验证过程介绍如下：GAPDH的引物序列来自一片外文文献：上游：AATGCATCCTGCACCACCAA下游：GTAGCCATATTCATTGTCATA所得片断为516bps。首先在pubmedline中查到GAPDH的mRNA序列，如图然后打开primer 5.0，点file→new→DNA sequence,将上面
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