alomone labs特约代理商|ACC-002 Anti

Antibody Purification

  Antibody Purification This protocol includes an ammonium sulfate cut, affigel blue chromatography and affinity chromatography.   Solutions Affigel Blue Prewash 0.1 M acetic acid 5.7 ml glacial acetic acid

Purification Of Anti Peptide Antibody

antibody that could contribute to staining background. The proportion in each pool varies with the peptide immunogen. The quality of the anti-peptide serum seems to increase with multiple boosts - the first bleed may be feeble.  

Whole mount antibody staining of zebrafish embryos for markers of segmentation

, 10 minutes each. 3. Incubate overnight at 4oC (cold room) in 0.5 ml primary antibody in 0.2% saponin in PBS. Primary antibodies: (A) znp-1 @ 1/2000 (primary motoneurons) (B)F6@ 1/500 (somite boundaries) (C) no antibody (Control)