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文献和实验Measurement of Chloride Movement in Neuronal Preparations
Literature Cited Biwersi, J. and Verkman, A.S. 1991. Cell‐permeable fluorescent indicator for cytosolic chloride. Biochemistry 30:7879‐7883
Competent cells, calcium chloride method, E. coli, description
(4000-5000 rpm for 5 min or 5000 x g for 5 min) at 4 C Keep the cells ice cold in all further steps. Resuspend the cells in 1/2 culture volume of 0.1 M ice-cold CaCl2. Hold on ice for minimum of 30 min, prefably 1 - 2 h
Cetyltrimethylammonium bromide (CTAB) Plant DNA Extraction
pipetting. 16. Add 100 μl of 3 M Sodium Acetate. Divide the sample evenly into two microcentrifuge tubes. 17. Add an equal volume of Phenol to each tube, mix well, centrifuge to separate the phases (1,000 X g), and save the aqueous phase (upper
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