bio-rad特约代理商|161-0374 蛋白marker

SDS-PAGE Western Blotting Protocols

cells or fruiting bodies. Klett 100 cells are at a density of 5E8. 2. Pellet at RT for 1-2’ and remove s/n. 3. Extract protein by SDS/Urea Method, TCA-precipitation, or sonication. 4. Measure [protein] by Bradford (use 1-2 ul of each sample

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

. The cultures are then pelleted at ~2,000 RPM for 4 minutes and the pellet re-suspended in 500µl of cold lysis buffer on ice for 10 minutes and then centrifuged 5,000 RPM for 5 minutes at 4°C. The pellet is considered the nuclear fraction while the solution

【原创】【翻译】【共享】QIANGEN Plasmid Midi and Maxi Kits 试剂盒说明书

2）以检测QIAGEN树脂的DNA结合效率。 11、用2×10ml或2×30ml Buffer QC冲洗QIAGEN-tip。 让Buffer QC经重力作用流过QIAGEN-tip。第一遍冲洗足以去除大部分质粒DNA的污染物。第二遍冲洗在大量培养或菌株会产生大量碳水化合物时是非常必需的。 从冲洗液中取400ul或240ul样本，保存用于凝胶分析（样本3）。 12、用5ml或15ml Buffer QF洗脱DNA。 用10ml或30ml管收集洗脱液。不推荐使用聚碳酸酯离心管，因为聚碳酸