hyglos特约代理商|321001 EndoGrade®

RNA Purification Protocol for 100-200 mg of Animal Tissue

. Storage at -70°C. Fresh tissue can also be used. Work quickly and keep the sample on ice at all times. 2. Add 100-200 mg frozen ground tissue or fresh tissue to a 15 mL centrifuge tube containing 6 ml RCL Buffer. Homogenize the sample using 5-10

MHC I STREPTAMERS® 检测与分离抗原特异性 (CD8+ T) 细胞

浓度为 1x107cells/ 100 μl，从中取 1 - 2x106 cells； 2． 用去离子水将 10xBuffer IS 稀释为 1x，准备 3 ml 稀释后的 Buffer IS； 3．取 1ul Strep-Jactin®PE 或 Strep-Tactin®APC，0.8μl MHC I-Strep，与 1x Buffer IS 混匀为 10ul 的终体积，冰上孵育 45 分钟； 4．将上一步孵育完的 Streptamers 复合物加入到细胞悬液中轻轻吹打混匀，冰上孵育 45 分钟

RNA Purification Protocol for 0.1-2.5 mg of Animal Tissue

. Fresh tissue can also be used. Work quickly and keep the sample on ice at all times. 2. Add 0.1-2.5 mg frozen ground tissue or fresh tissue to a 0.5 mL microtube containing 100ul RCL Buffer. Homogenize throughly using a microfuge tube pestle