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上海玉博生物科技有限公司
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文献和实验RNA Purification Protocol for 100-200 mg of Animal Tissue
. Storage at -70°C. Fresh tissue can also be used. Work quickly and keep the sample on ice at all times. 2. Add 100-200 mg frozen ground tissue or fresh tissue to a 15 mL centrifuge tube containing 6 ml RCL Buffer. Homogenize the sample using 5-10
MHC I STREPTAMERS® 检测与分离抗原特异性 (CD8+ T) 细胞
浓度为 1x107cells/ 100 μl,从中取 1 - 2x106 cells; 2. 用去离子水将 10xBuffer IS 稀释为 1x,准备 3 ml 稀释后的 Buffer IS; 3.取 1ul Strep-Jactin®PE 或 Strep-Tactin®APC,0.8μl MHC I-Strep,与 1x Buffer IS 混匀为 10ul 的终体积,冰上孵育 45 分钟; 4.将上一步孵育完的 Streptamers 复合物加入到细胞悬液中轻轻吹打混匀,冰上孵育 45 分钟
RNA Purification Protocol for 0.1-2.5 mg of Animal Tissue
. Fresh tissue can also be used. Work quickly and keep the sample on ice at all times. 2. Add 0.1-2.5 mg frozen ground tissue or fresh tissue to a 0.5 mL microtube containing 100ul RCL Buffer. Homogenize throughly using a microfuge tube pestle
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