cell signaling特约代理商|5872 Prote

Co-Expression of human DNA primase p49-p58 subunits

on cell pellet obtained from 4L growth)and add protease inhibitors.Use ROCHE EDTA-free cocktail tablets-1 tablet/10ml lysis buffer (It's important that all your solutions are EDTA-free because EDTA is incompatable w/Ni-NTA resin).Rotate ~5 min 4ºC

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

.   9. Wash twice with 10 mL of PBS containing protease inhibitor cocktail. Working on ice, pipette gently to make a single-cell suspension. After resuspending the second time, count the cells before centrifugation.   10

Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer

of most protein tyrosine phosphatases) Protease inhibitor cocktail (proprietary mixture) 1X PBS Cell scraper 1.5 ml microcentrifuge tube Centrifuge Pipette man Filtered pipette tips Kimwipes Waste beaker