ROTH特约代理商|EKN7.1 Pasteur pipet

PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID

    Dissecting microscope;       Acid Tyrode's (e.g. Sigma: T1788);       Sterile plastic Petri dishes (100 x 15 mm);       M2 and KSOM medium in 1 ml syringes;       9'' Pasteur pipettes;       Mouth or Finger controlled pipette. Method: Place

Cell Cultures

Broth culture of E. coli Tubes of nutrient broth (9.9 ml each) Nutrient agar plates Sterile transfer pipettes (1.0 ml) Quebec colony counter (Optional)   Procedure 1. Obtain a broth culture of E. coli and carefully mix

PRODUCTION OF ES CELL CHIMERAS BY AGGREGATION WITH EIGHT-CELL STAGE EMBRYOS

Sterile plastic Petri dishes (100 x 15 mm);  M2 and KSOM medium in 1 ml syringes;  9'' Pasteur pipettes;  Mouth or Finger controlled pipette.  Method:  1. Place a few drops of M2, KSOM and acid Tyrode's