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文献和实验Modification of the 5′ Terminus of Oligonucleotides for Attachment of Reporter and Conjugate Groups
Abstract Table of Contents Figures Literature Cited Abstract Reporter and conjugate groups
IMMUNOSCREENING OF WITH ANTIBODY PROBES
. Incubate these membrane sections with secondary antiserum for 1 hour at room temperature (For detecting human antibodies, we use Sigma #A-3150: goat anti-human IgG alkaline phosphatase conjugate, diluted 1:1000) Wash membranes in TBST for 5 minutes
IMMUNOSCREENING OF LAMBDA-GT11 BACTERIOPHAGE LIBRARIES WITH ANTIBODY PROBES
dishes of lysed E. coli to reduce anti-E. coli antibody background. These antibodies are present in all human sera, rabbit sera, and rodent sera. For each Petri dish containing solidified LB agar, mix 0.6ml of the Y1090 overnight-saturated culture with 30
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