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上海玉博生物科技有限公司
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文献和实验问: 1.DEAE FAST FLOW经过不同盐浓度洗脱后,在用初始缓冲液平衡柱子过程中,缓冲液的体积和平衡时间与什么有关?我要的蛋白等电点是4.5,用的是PH7.5的20mMTris-HCl缓冲液平衡,而且我用的是5ML的预装柱,洗脱后平衡得需要大约120ML的缓冲液,我看说明书写的只需5-6个柱体积,这是为什么呢? 2.如果自己装的DEAE FAST
Vybrant® DyeCycle Green and Orange Stains
, bound to DNA. Materials Required but Not Provided Cells and culture medium Flow cytometer tubes Caution The hazards posed by these stains have not been fully investigated. Since Vybrant® DyeCycle™ Green and Orange
have been used to obtain enriched or purified cell populations, including flow cell sorting. Current protocols are usually time-consuming and may imply loss of short-lived RNAs, which is undesirable for expression profiling. We describe an optimized method
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