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文献和实验The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
reagent; BD Biosciences 555899) (for Protocol 4) Buffer for cell staining and cell sorting (for Protocol 4; see Step 72) Complement, reconstituted from rabbit (Cedarlane CL3331) (for Protocol 3) ESC medium (for Protocols 1, 2) Freezing
Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
of helper phage in the cell. Helper phage can be easily removed by increasing the ratio of bacteria to phage during the plating in step 6 above. References: Sparks, A.B., Hoffman, N.G., McConnell, S.J., Fowlkes, D.M., and Kay, B.K
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