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文献和实验Synchronization of Bacillus subtilis Cells by Spore Germination and Outgrowth
This protocol defines conditions under which the germination of spores can be used to synchronize Bacillus subtilis cells, utilizing the time-ordered sequence of events taking place during the transition from spore to vegetative cells
makes this easier to enforce.Keep all work surfaces free of clutter.Correctly label reagents including flasks, medium and ampules with contents and date of preparation.Only handle one cell line at a time. This common-sense point will reduce
Subculture of Suspension Cell Lines
pellets in a small volume (10-20mls) of fresh culture medium. Pool the cell suspensions. Count the cells. Pipette the required number of cells to a new labeled flask and dilute to the required volume using fresh medium (refer to ECACC Cell Line Data Sheet
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