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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
salt NBT, (Altman, 1972) can be used in conjunction with X-Phos as the final electron acceptor for the indoxyl dimerization reaction. When reduced, NBT forms a purple precipitate. Of the cloned phosphatases, the human placental alkaline
Determination of Molecular Size by Zonal Sedimentation Analysis on Sucrose Density Gradients
from of Standard Proteins Support Protocol 3: Determination of the Value of ST,m Support Protocol 4: Determination of the Value of Partial Specific Volume (υ) Support Protocol 5: Determination
The sedimentation coefficient, S , can be determined in an analytical ultracentrifuge. This was a standard part of the characterization of proteins in the 1940s and 1950s, and values of S 20,w (sedimentation coefficient standardized to 20°C in water) are collected
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