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上海玉博生物科技有限公司
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文献和实验Harvesting lymphocytes from Peripheral Blood
cells) ・ Incubate at 4°C for 30 minutes ・ Add 2ml of cold lytic buffer solution to each specimen for 2 minutes � gently shake them ・ After 2 minutes fill the facs tubes with PBS ・ Spin at 1000rpm (450g) for 10
DIRECT AND SHORT-TERM PROCEDURE FOR HARVESTING BONE MARROW CHROMOSOMES
for trisomy 12. B. For all ALL, be sure to look at non-GCT cultures also. VI. Solutions: Colcemid working solution: 10 mg Colcemid in 250 ml dH2O, sterile filtered, store at 4 C. Complete RPMI-1640: 100 ml RPMI-1640, 20 ml Fetal calf serum
BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method
antibiotic. After incubating at 37 degrees C for 8-10 hours with 250 rpm shaking, the culture is transferred to a 500 ml flask containing 200 ml of the same medium and incubated for 8-10 hours under the same conditions. After harvesting the cells
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